Objective To investigate the species of mosquitoes and the distribution of arboviruses carried by the mosquitoes in Hexi Corridor of Gansu province, China in 2011. Methods Mosquito samples were collected from 6 p.m. to 6 a.m. in the next day with UV lamps. After being identified, the samples were preserved in liquid nitrogen and then transported to a laboratory for examination. Viruses were isolated from the samples by tissue culture, and the virus isolates were identified by serological and molecular biological methods. Homology and phylogenetic analysis was performed on the nucleotide sequences of isolated viruses by bioinformatics. Results A total of 24 028 mosquitoes, belonging to 6 species and 3 genera, were collected in seven counties or cities along Hexi Corridor in August 2011. Among the collected mosquitoes, Aedes vexans was the most abundant species, accounting for 32.80% (7880/24 028), and Culex pipiens pallens accounted for 30.26% (7272/24 028). One virus strain (GS11-155) that could induce pathological changes of two types of cells (BHK-21 and C6/36 cells) was isolated by cell culture. Genes of Culex flavivirus (CxFV), Liaoning virus (LNV), and Getah virus (GETV) were detected in 32 batches of mosquito samples, and in 11 of the 32 batches, 1 had CxFV, 3 had GETV, and 7 had LNV, according to nucleotide sequencing. Conclusion Ae. vexans was the dominant species of mosquitoes in Hexi Corridor of Gansu province, China. The mosquitoes in Hexi Corridor carry several types of arboviruses, including the CxFV, GETV, and LNV

【Abstract】 Objective To learn the infection status of Lyme disease in Diebu county, the change of natural focus and the situation of the host infected by Lyme disease. Methods The antibody of Borrelia burgdoferi in the crowd serum was detected by immunofluorescence, and the spirochete DNA was tested with PCR. Results A total of 522 serums were sampled from the people in 5 forestry centres and 7 villages of Dieby county, and the antibodies of B.burgdoferi were positive in 57 serums with the positive rate of 10.92%. Sixty nine species of rat-shapes were captured, and the average capture rate was 15.33%. Among them, there were 25 Niniventer confucianus Hodgsons,18 Rattus norvegicus Berkenhouts, 15 Apodemus specious Temminck, 9 Apodemus specious Linnaeus, 1 Mus musculus and 1 Apodemus agrarius Pallas, which accounted for 36.23%, 26.09%, 21.74%, 13.04%, 1.45% and 1.45%, respectively. Six spleens and five kidnies were positive in 62 rats?spleens and 66 rat-kidnies, and the positive rate was 15.15%（10/66）. The positive samples included  6  R.niviventer  Hodgsons,  2  R.norvegicus  Berkenhouts, 1 A.specious Temminck and 1 A.specious Linnaeus three strains of B.burgdoferi were isolated from rats kidnies and bladders with BSK culture medium. Conclusion The natural foci of Lyme disease were widely distributed in Diebu county, and the infection rate in the crowd was higher.